rabbit anti-npc1 Search Results


npc1 antibody a4795  (ABclonal Biotechnology)
90
ABclonal Biotechnology npc1 antibody a4795
The role of <t>NPC1</t> in DEHV entry into cells. (A) Western blotting analysis of NPC1 expression levels in different experimental conditions. (B) Infection efficiencies of DEHV pseudovirus in wild-type HEK293T cells, <t>NPC1-knockout</t> HEK293T (HEK293T-NPC1-KO) cells, and HEK293T-NPC1-KO cells transiently expressing human NPC1 (hNPC1). The relative ratios of luciferase values in wild-type HEK293T cells and HEK293T-NPC1-KO cells are shown. (C) Expression of NPC1 from different species in HEK293T-NPC1-KO cells. Cells were transfected with 10 µg of vector encoding NPC1 proteins from various species; GFP expression was analyzed using flow cytometry as a proxy for NPC1 expression. (D) Fluorescence values reflecting DEHV pseudovirus entry into HEK293T-NPC1-KO cells transfected with vectors encoding NPC1 proteins from different species. Data represent four independent biological replicates ( n = 4).
Npc1 Antibody A4795, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/npc1 antibody a4795/product/ABclonal Biotechnology
Average 90 stars, based on 1 article reviews
npc1 antibody a4795 - by Bioz Stars, 2026-06
90/100 stars
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rabbit anti- npc1 polyclonal antibody  (Cusabio)
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Rabbit anti-Human NPC1 Polyclonal Antibody
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The role of NPC1 in DEHV entry into cells. (A) Western blotting analysis of NPC1 expression levels in different experimental conditions. (B) Infection efficiencies of DEHV pseudovirus in wild-type HEK293T cells, NPC1-knockout HEK293T (HEK293T-NPC1-KO) cells, and HEK293T-NPC1-KO cells transiently expressing human NPC1 (hNPC1). The relative ratios of luciferase values in wild-type HEK293T cells and HEK293T-NPC1-KO cells are shown. (C) Expression of NPC1 from different species in HEK293T-NPC1-KO cells. Cells were transfected with 10 µg of vector encoding NPC1 proteins from various species; GFP expression was analyzed using flow cytometry as a proxy for NPC1 expression. (D) Fluorescence values reflecting DEHV pseudovirus entry into HEK293T-NPC1-KO cells transfected with vectors encoding NPC1 proteins from different species. Data represent four independent biological replicates ( n = 4).

Journal: Microbiology Spectrum

Article Title: Development of a neutralization assay and bioluminescent imaging mouse model for Dehong virus (DEHV) using a pseudovirus system

doi: 10.1128/spectrum.01557-24

Figure Lengend Snippet: The role of NPC1 in DEHV entry into cells. (A) Western blotting analysis of NPC1 expression levels in different experimental conditions. (B) Infection efficiencies of DEHV pseudovirus in wild-type HEK293T cells, NPC1-knockout HEK293T (HEK293T-NPC1-KO) cells, and HEK293T-NPC1-KO cells transiently expressing human NPC1 (hNPC1). The relative ratios of luciferase values in wild-type HEK293T cells and HEK293T-NPC1-KO cells are shown. (C) Expression of NPC1 from different species in HEK293T-NPC1-KO cells. Cells were transfected with 10 µg of vector encoding NPC1 proteins from various species; GFP expression was analyzed using flow cytometry as a proxy for NPC1 expression. (D) Fluorescence values reflecting DEHV pseudovirus entry into HEK293T-NPC1-KO cells transfected with vectors encoding NPC1 proteins from different species. Data represent four independent biological replicates ( n = 4).

Article Snippet: Samples were incubated with DEHV mouse serum (1:1,000 dilution, followed by an anti-mouse secondary antibody) or NPC1 antibody (Abclonal, A4795 , followed by an anti-rabbit secondary antibody).

Techniques: Western Blot, Expressing, Infection, Knock-Out, Luciferase, Transfection, Plasmid Preparation, Flow Cytometry, Fluorescence